| Long name: |
FITC Conjugated, MEK5(Ser311+Thr315) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MEK5 Ser311+Thr315 |
| Specificity: |
This is a highly specific antibody against MEK5 Ser311+Thr315 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser311+Thr315 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser311+Thr315 |
| Gene ID number: |
5607 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
(MAP kinase kinase family), It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, It is not phosphorylated by RAFA, MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle, RAFB or RAFC and it may interact with GTPases such as CDC42, The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation, This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, This kinase specifically interacts with and activates MAPK7/ERK5, and not with another MAP kinase like P38, as well as by atypical protein kinase C isoforms (aPKCs), MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
MEK5 MAPKK5 HsT17454 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MEK5(Ser311+Thr315) Conjugated |
| Short name: |
FITC Conjugated, MEK5(Ser311+Thr315) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, MEK5(Ser311+Thr315) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6845 |
| Gene: |
MAP2K5 |
More about : MAP2K5 |
| Long gene name: |
mitogen-activated protein kinase kinase 5 |
| Synonyms gene: |
PRKMK5 |
| Locus: |
15q23 |
| Discovery year: |
1997-11-11 |
| GenBank acession: |
U25265 |
| Entrez gene record: |
5607 |
| Pubmed identfication: |
7759517 |
| RefSeq identity: |
NM_145162 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000133264 |