| Long name: |
FITC Conjugated, Histone H3 (Ser28) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Histone H3 Ser28 |
| Specificity: |
This is a highly specific antibody against Histone H3 Ser28 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser28 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser28 |
| Gene ID number: |
3020 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Covalent modifications of the canonical core histones, H1, H2B, H3, Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, The chromatin fibre is further compacted through the interaction of a linker histone, The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways, and H4), and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions, including acetylation, methylation, phosphorylation, with the DNA between the nucleosomes to form higher order chromatin structures, Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
H3 histone family member E pseudogene, H31_HUMAN, H3F, H3F3, H3S, HIST3H3, Histone H3, Histone H3 3 pseudogene, Histone H3-I/H3-II, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone H3S28, Histone H3Ser28, Histone H3p-Ser28, Major histone H3, Histone H3Ser28 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Histone H3 (Ser28) Conjugated |
| Short name: |
FITC Conjugated, Histone H3 (Ser28) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Histone H3 (Ser28) (Antibody to) |
| Alternative technique: |
antibodies |