| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Histone H3 Ser28 |
| Specificity: |
This antibody reacts specifically with Histone H3 (Ser28) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser28 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser28 |
| Gene ID: |
3020 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Covalent modifications of the canonical core histones, H1, H2B, H3, Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, The chromatin fibre is further compacted through the interaction of a linker histone, The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways, and H4), and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions, including acetylation, methylation, phosphorylation, with the DNA between the nucleosomes to form higher order chromatin structures, Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
H3 histone family member E pseudogene, H31_HUMAN, H3F, H3F3, H3S, HIST3H3, Histone H3, Histone H3 3 pseudogene, Histone H3-I/H3-II, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone H3S28, Histone H3Ser28, Histone H3p-Ser28, Major histone H3, Histone H3Ser28 |
| Also known as: |
Histone H3 (Ser28) Polyclonal Antibody |
| Other name: |
Anti-Histone H3 (Ser28) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Histone H3 (Ser28) |
| Short name: |
Anti-Histone H3 (Ser28) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Histone H3 (Ser28) (polyclonal) |
| Alternative technique: |
polyclonals |