| Long name: |
FITC Conjugated, Fast skeletal Myosin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Fast skeletal Myosin |
| Specificity: |
This antibody may recognize myosin light chain 5, myosin light chain 7, or myosin regulatory light chain 10, This is a highly specific antibody against Fast skeletal Myosin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
120-170/170 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human MRLC2 |
| Gene ID number: |
29895 |
| Swiss Prot: |
Q96A32 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase, Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, Myosin heavy chains, Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle, Three general classes of Myosin have been cloned: smooth muscle Myosins, Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits, contain Actin-activated ATPase activity which generates the motor function of Myosin, striated muscle Myosins and non-muscle Myosins , ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements, which are encoded by the MYH gene family, Myosin is a highly conserved |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Fast skeletal myosin light chain 2, HUMMLC2B, MLC2B, MYL11, MYLPF, Myosin regulatory light chain 2, skeletal muscle isoform, MRLC2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Fast skeletal Myosin Conjugated |
| Short name: |
FITC Conjugated, Fast skeletal Myosin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Fast skeletal Myosin (Antibody to) |
| Alternative technique: |
antibodies |