| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Fast skeletal Myosin |
| Specificity: |
myosin light chain 7, or myosin regulatory light chain 10 Fast skeletal Myosin, This antibody may recognize myosin light chain 5 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MRLC2 |
| Gene ID: |
29895 |
| Swiss Prot: |
Q96A32 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase, Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, Myosin heavy chains, Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle, Three general classes of Myosin have been cloned: smooth muscle Myosins, Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits, contain Actin-activated ATPase activity which generates the motor function of Myosin, striated muscle Myosins and non-muscle Myosins , ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements, which are encoded by the MYH gene family, Myosin is a highly conserved |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MRLC2 HUMMLC2B MYL11 |
| Also known as: |
Fast skeletal Myosin Polyclonal Antibody |
| Other name: |
Anti-Fast skeletal Myosin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Fast skeletal Myosin |
| Short name: |
Anti-Fast skeletal Myosin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Fast skeletal Myosin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
29824 |
| Gene: |
MYLPF |
More about : MYLPF |
| Long gene name: |
fast skeletal muscle , myosin light chain, phosphorylatable |
| Synonyms name: |
light chain 11, myosin, regulatory myosin regulatory light chain 2 |
| Locus: |
16p11, 2 |
| Discovery year: |
2009-03-06 |
| GenBank acession: |
AF363061 |
| Entrez gene record: |
29895 |
| Pubmed identfication: |
6179945 |
| RefSeq identity: |
NM_013292 |
| Classification: |
Myosin light chains EF-hand domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132399 |