| Long name: |
FITC Conjugated, Sphingomyelin Synthase 1 Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Sphingomyelin Synthase 1 |
| Specificity: |
This is a highly specific antibody against Sphingomyelin Synthase 1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
50-100/419 |
| Subcellular location: |
Intracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Sphingomyelin Synthase 1 |
| Gene ID number: |
259230 |
| Swiss Prot: |
Q86VZ5 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, Major form in macrophages, May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide, Required for cell growth in certain cell types such as HeLa cells, Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide, elevated temperature and exogenously supplied sphingolipids, on to the primary hydroxyl of ceramide, osmotic stress, phosphatidylcholine, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate, sphingomyelin, through transfer of the phosphatidyl head group, Sphingomyelin synthases synthesize the sphingolipid |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
MOB1, Medulla oblongata-derived protein, Phosphatidylcholine:ceramide cholinephosphotransferase 1, Protein Mob, SGMS1, SMS1, Sphingomyelin synthase 1, TMEM23, Transmembrane protein 23, hmob33, MOB |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Sphingomyelin Synthase 1 Conjugated |
| Short name: |
FITC Conjugated, Sphingomyelin Synthase 1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Sphingomyelin Synthase 1 (Antibody to) |
| Alternative technique: |
antibodies |