| Long name: |
RPA32 Primary Polyclonal Antibody |
| Also known as: |
RPA32 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
RPA32 |
| Specificity: |
This is a highly specific antibody against RPA32 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
110-160/270 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human RPA32/RPA2 |
| Gene ID number: |
6118 |
| Swiss Prot: |
P15927 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Also recruits SMARCAL1/HARP, Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair, In the cellular response to DNA damage, It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage, It prevents their reannealing and in parallel, May also play a role in telomere maintenance, Plays also a role in base excision repair (BER) probably through interaction with UNG, Thereby, Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control, Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response, binds and stabilizes single-stranded DNA intermediates, it plays an essential role both in DNA replication and the cellular response to DNA damage, recruits and activates different proteins and complexes involved in DNA metabolism, that form during DNA replication or upon DNA stress, the RPA complex controls DNA repair and DNA damage checkpoint activation, to sites of DNA damage, which is involved in replication fork restart, As part of the heterotrimeric replication protein A complex (RPA/RP-A) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
RF-A protein 2, RP-A p32, RP-A p34, RPA2, RPA32, RPA34, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, REPA2 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RPA32 |
| Short name: |
RPA32 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
RPA32 (Antibody to) |
| Alternative technique: |
antibodies |