| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RPA32 |
| Specificity: |
This antibody reacts specifically with RPA32 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human RPA32/RPA2 |
| Gene ID: |
6118 |
| Swiss Prot: |
P15927 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also recruits SMARCAL1/HARP, Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair, In the cellular response to DNA damage, It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage, It prevents their reannealing and in parallel, May also play a role in telomere maintenance, Plays also a role in base excision repair (BER) probably through interaction with UNG, Thereby, Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control, Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response, binds and stabilizes single-stranded DNA intermediates, it plays an essential role both in DNA replication and the cellular response to DNA damage, recruits and activates different proteins and complexes involved in DNA metabolism, that form during DNA replication or upon DNA stress, the RPA complex controls DNA repair and DNA damage checkpoint activation, to sites of DNA damage, which is involved in replication fork restart, As part of the heterotrimeric replication protein A complex (RPA/RP-A) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RF-A protein 2, RP-A p32, RP-A p34, RPA2, RPA32, RPA34, Replication factor A protein 2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, REPA2 |
| Also known as: |
RPA32 Polyclonal Antibody |
| Other name: |
Anti-RPA32 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RPA32 |
| Short name: |
Anti-RPA32 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RPA32 (polyclonal) |
| Alternative technique: |
polyclonals |