| Long name: |
FITC Conjugated, ATG1/ULK1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ATG1/ULK1 |
| Specificity: |
This is a highly specific antibody against ATG1/ULK1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1004-1050/1050 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human ATG1/ULK1 |
| Gene ID number: |
8408 |
| Swiss Prot: |
O75385 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, May phosphorylate ATG13/KIAA652 and RPTOR, PRKAB2 and PRKAG1, Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR, Plays a role early in neuronal differentiation and is required for granule cell axon formation, however such data need additional evidences, leading to negatively regulate AMPK activity, the precursors of autophagosomes, Serine/threonine-protein kinase involved in autophagy in response to starvation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ATG1 ATG1A |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ATG1/ULK1 Conjugated |
| Short name: |
FITC Conjugated, ATG1/ULK1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ATG1/ULK1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12558 |
| Gene: |
ULK1 |
More about : ULK1 |
| Long gene name: |
unc-51 like autophagy activating kinase 1 |
| Synonyms gene name: |
elegans) , elegans)-like kinase 1 unc-51-like kinase 1 (C, unc-51 (C |
| Synonyms name: |
ATG1 autophagy related 1 homolog (S, cerevisiae) |
| Locus: |
12q24, 33 |
| Discovery year: |
1998-09-03 |
| GenBank acession: |
AF045458 |
| Entrez gene record: |
8408 |
| Pubmed identfication: |
9693035 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000168052 |