| Long name: |
FITC Conjugated, PLK1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PLK1 |
| Specificity: |
This is a highly specific antibody against PLK1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
240-290/603 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human PLK1 |
| Gene ID number: |
5347 |
| Swiss Prot: |
P53350 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies, PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa, PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase, PLK1 is modified by phosphorylation at Threonine 210, PLK1 may also be required for cell division, cdc5/polo subfamily, PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PLK1 Conjugated |
| Short name: |
FITC Conjugated, PLK1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PLK1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9077 |
| Gene: |
PLK1 |
More about : PLK1 |
| Long gene name: |
polo like kinase 1 |
| Synonyms gene: |
PLK |
| Synonyms gene name: |
polo-like kinase (Drosophila) polo-like kinase 1 |
| Locus: |
16p12, 2 |
| Discovery year: |
1994-07-25 |
| Entrez gene record: |
5347 |
| Pubmed identfication: |
8127874 |
| RefSeq identity: |
NM_005030 |
| Havana BLAST/BLAT: |
OTTHUMG00000096984 |