| Long name: |
FITC Conjugated, PAK4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PAK4 |
| Specificity: |
This is a highly specific antibody against PAK4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human PAK4 |
| Gene ID number: |
10298 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, and lipids, and transcription, apoptosis, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PAK4 Conjugated |
| Short name: |
FITC Conjugated, PAK4 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PAK4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16059 |
| Gene: |
PAK4 |
More about : PAK4 |
| Long gene name: |
p21 (RAC1) activated kinase 4 |
| Synonyms gene name: |
p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4 |
| Locus: |
19q13, 2 |
| Discovery year: |
2001-07-12 |
| GenBank acession: |
AJ011855 |
| Entrez gene record: |
10298 |
| Pubmed identfication: |
9822598 10461188 |
| Havana BLAST/BLAT: |
OTTHUMG00000182800 |