| Long name: |
FITC Conjugated, PAK4/5(Ser99) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PAK4/5 Ser99 |
| Specificity: |
This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against PAK4/5 Ser99 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser99 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
80-120/591 |
| Subcellular location: |
Mitochondrion, Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99 |
| Gene ID number: |
10298, 57144 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues, All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphorylates CTNND1, Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, and lipids, and transcription, apoptosis, cell migration, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, probably to regulate cytoskeletal organization and cell morphology, proliferation or cell survival, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PAK4/5(Ser99) Conjugated |
| Short name: |
FITC Conjugated, PAK4/5(Ser99) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PAK4/5(Ser99) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16059 |
| Gene: |
PAK4 |
More about : PAK4 |
| Long gene name: |
p21 (RAC1) activated kinase 4 |
| Synonyms gene name: |
p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4 |
| Locus: |
19q13, 2 |
| Discovery year: |
2001-07-12 |
| GenBank acession: |
AJ011855 |
| Entrez gene record: |
10298 |
| Pubmed identfication: |
9822598 10461188 |
| Havana BLAST/BLAT: |
OTTHUMG00000182800 |