| Long name: |
Cy5 Conjugated, PAK4/5(Ser99) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PAK4/5 Ser99 |
| Specificity: |
This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against PAK4/5 Ser99 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser99 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
80-120/591 |
| Subcellular location: |
Mitochondrion, Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99 |
| Gene ID number: |
10298, 57144 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues, All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphorylates CTNND1, Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, and lipids, and transcription, apoptosis, cell migration, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, probably to regulate cytoskeletal organization and cell morphology, proliferation or cell survival, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
625, 650nm/670nm |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PAK4/5(Ser99) Conjugated |
| Short name: |
Conjugated, PAK4/5(Ser99) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy5 |
| Alternative name: |
cyanine 5 coupled, PAK4/5(Ser99) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16059 |
| Gene: |
PAK4 |
More about : PAK4 |
| Long gene name: |
p21 (RAC1) activated kinase 4 |
| Synonyms gene name: |
p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4 |
| Locus: |
19q13, 2 |
| Discovery year: |
2001-07-12 |
| GenBank acession: |
AJ011855 |
| Entrez gene record: |
10298 |
| Pubmed identfication: |
9822598 10461188 |
| Havana BLAST/BLAT: |
OTTHUMG00000182800 |