| Long name: |
Cy3 Conjugated, Rictor (Thr1135) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Rictor Thr1135 |
| Specificity: |
This phosphorylation site is homologous to that of Thr1135 in Mouse and Thr1120 in Rat, This is a highly specific antibody against Rictor Thr1135 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr1135 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1100-1150/1732 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Rictor around the phosphorylation site of Thr1135 |
| Gene ID number: |
253260 |
| Swiss Prot: |
Q6R327 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Plays an essential role in embryonic growth and development, but, in contrast to mTORC1, mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657', mTORC2 is activated by growth factors, mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, mTORC2 promotes the serum-induced formation of stress-fibers or F-actin, mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422', mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors, seems to be nutrient-insensitive, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation, which regulates cell growth and survival in response to hormonal signals, Subunit of mTORC2 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
AVO3, AVO3 homolog, KIAA1999, RICTOR, Rapamycin-insensitive companion of mTOR, hAVO3, PIA |
| Properties: |
1 of the Rictor (Thr1135) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Rictor (Thr1135) Conjugated |
| Short name: |
Conjugated, Rictor (Thr1135) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, Rictor (Thr1135) (Antibody to) |
| Alternative technique: |
antibodies |