| Long name: |
FITC Conjugated, c-Fos (Thr325) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
c-Fos Thr325 |
| Specificity: |
This phosphorylation site is homologous to Thr325 in Mouse and Rat, This is a highly specific antibody against c-Fos Thr325 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr325 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
300-350/380 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325 |
| Gene ID number: |
2353 |
| Swiss Prot: |
P01100 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites, Has a critical function in regulating the development of cells destined to form and maintain the skeleton, In growing cells, In the heterodimer, It is thought to have an important role in signal transduction, On TGF-beta activation, This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum, activates phospholipid synthesis, cell proliferation and differentiation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling, possibly by activating CDS1 and PI4K2A, Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
AP-1, C-FOS, Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene c-Fos, p55 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
c-Fos (Thr325) Conjugated |
| Short name: |
FITC Conjugated, c-Fos (Thr325) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, c-FBJ murine osteosarcoma viral oncogene homolog (Thr325) (Antibody to) |
| Alternative technique: |
antibodies |