| Long name: |
ALEXA FLUOR 488 Conjugated, Btk (Ser180) Polyclonal Antibody |
| Also known as: |
Anti-Btk Ser180 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Btk Ser180 |
| Specificity: |
This is a highly specific antibody against Btk Ser180 |
| Modification: |
Phosphorylation |
| Modification Site: |
Ser180 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
160-200/659 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Btk around the phosphorylation site of Ser180 [GS(p-S)HR] |
| Gene ID Number: |
695 |
| Swiss Prot: |
Q06187 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
ARID3A and NFAT are other transcriptional target of BTK, After BCR engagement and activation at the plasma membrane, BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways, BTK has a dual role in the regulation of apoptosis, BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B, BTK is required for the formation of functional ARID3A DNA-binding complexes, BTK plays also a critical role in transcription regulation, Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation, Especially, GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression, Induces the activity of NF-kappa-B, PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK, Plays an important role in the function of immune cells of innate as well as adaptive immunity, The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense, There is however no evidence that BTK itself binds directly to DNA, Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR, Within the TLR pathway, as a component of the Toll-like receptors (TLR) pathway, differentiation and signaling, followed by activation of the protein kinase C (PKC) family members, igniting the downstream signaling pathway through calcium mobilization, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation, is a critical molecule in regulating TLR9 activation in splenic B-cells, phosphorylates PLCG2 at several sites, which is involved in regulating the expression of hundreds of genes, Non-receptor tyrosine kinase indispensable for B lymphocyte development |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Synonyms: |
AGMX1, ATK, Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, BPK, BTK, Bruton tyrosine kinase, IMD1, PSCTK1, Tyrosine-protein kinase BTK, XLA, AT |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the Btk (Ser180) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
Btk (Ser180) |
| Short name: |
Btk (Ser180) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, Bruton agammaglobulinemia tyrosine kinase (Ser180) (Antibody to) |
| Alternative technique: |
antibodies |