| Long name: |
FITC Conjugated, Btk (Ser180) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Btk Ser180 |
| Specificity: |
This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against Btk Ser180 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser180 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
160-200/659 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Btk around the phosphorylation site of Ser180 [GS(p-S)HR] |
| Gene ID number: |
695 |
| Swiss Prot: |
Q06187 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ARID3A and NFAT are other transcriptional target of BTK, After BCR engagement and activation at the plasma membrane, BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways, BTK has a dual role in the regulation of apoptosis, BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B, BTK is required for the formation of functional ARID3A DNA-binding complexes, BTK plays also a critical role in transcription regulation, Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation, Especially, GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression, Induces the activity of NF-kappa-B, PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK, Plays an important role in the function of immune cells of innate as well as adaptive immunity, The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense, There is however no evidence that BTK itself binds directly to DNA, Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR, Within the TLR pathway, as a component of the Toll-like receptors (TLR) pathway, differentiation and signaling, followed by activation of the protein kinase C (PKC) family members, igniting the downstream signaling pathway through calcium mobilization, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation, is a critical molecule in regulating TLR9 activation in splenic B-cells, phosphorylates PLCG2 at several sites, which is involved in regulating the expression of hundreds of genes, Non-receptor tyrosine kinase indispensable for B lymphocyte development |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
AGMX1, ATK, Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, BPK, BTK, Bruton tyrosine kinase, IMD1, PSCTK1, Tyrosine-protein kinase BTK, XLA, AT |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Btk (Ser180) Conjugated |
| Short name: |
FITC Conjugated, Btk (Ser180) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Bruton agammaglobulinemia tyrosine kinase (Ser180) (Antibody to) |
| Alternative technique: |
antibodies |