| Long name: |
FITC Conjugated, Tap1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Tap1 |
| Specificity: |
This is a highly specific antibody against Tap1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
525-575/808 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human Tap1/ABCB2 |
| Gene ID number: |
6890 |
| Swiss Prot: |
Q03518 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also acts as a molecular scaffold for the final stage of MHC class I folding, Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-1 protein, Inhibited by human adenovirus E3-19K glycoprotein, Inhibited by human cytomegalovirus US6 glycoprotein, Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, Nascent MHC class I molecules associate with TAP via tapasin, namely the binding of peptide, preventing MHC class I/TAP association, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules, which binds the TAP complex and acts as a tapasin inhibitor, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding, which blocks the peptide-binding site of TAP, Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ABC17, ABCB2, ATP-binding cassette sub-family B member 2, Antigen peptide transporter 1, D6S114E, PSF-1, PSF1, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, RING4, Really interesting new gene 4 protein, TAP1, TAP1*12N, TAP1N, Y3, APT1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Tap1 Conjugated |
| Short name: |
FITC Conjugated, Tap1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
adenosine triphosphate-binding cassette, fluorecein coupled, sub-family B (multi-drug-resistant/TAP) (Antibody to), transporter 1 |
| Alternative technique: |
antibodies |