| Long name: |
FITC Conjugated, Crk p38 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Crk p38 |
| Specificity: |
This is a highly specific antibody against Crk p38 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Crk p38 /CrkII |
| Gene ID number: |
1398 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Crk is believed to be a regulator of invasive responses because increased levels of the protein have been observed in multiple human cancers, CrkI and CrkII are produced from the same crk gene by alternative splicing, In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth, It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation, The two isoforms differ in their biological activities with CrkII having less transforming activity than CrkI, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction, Crk p38 (CrkII) is an isoform of the protein Crk, Crk p38 is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Adapter molecule crk, Avian sarcoma virus CT10 v crk oncogene homolog, CRK isoform 2, CRK isoform II, CRKII, CrkII, Proto oncogene C crk, p38, v crk avian sarcoma virus CT10 oncogene homolog, v crk sarcoma virus CT10 oncogene homolog, v crk sarcoma virus CT10 oncogene homolog avian, CRK |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Crk p38 Conjugated |
| Short name: |
FITC Conjugated, Crk p38 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Crk p38 (Antibody to) |
| Alternative technique: |
antibodies |