| Long name: |
FITC Conjugated, Ki-67 (Proliferation Marker) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Ki-67 proliferation Marker |
| Specificity: |
This is a highly specific antibody against Ki-67 proliferation Marker |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1250-1300/3256 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human Ki67 |
| Gene ID number: |
4288 |
| Swiss Prot: |
P46013 |
| Tested applications: |
IF(ICC), IF(IHC-P) |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
G2 and M phase), In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index, It is absent in resting (G0) cells, Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index), Ki67 is routinely used as a neuronal marker of cell cycling and proliferation, S, The correlation between low Ki67 index and histologically low grade tumours is strong, expressed by proliferating cells in all phases of the active cell cycle (G1, Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Antigen KI-67, MIB-, MIB-1, MKI67, PPP1R15, KIA |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Ki-67 (Proliferation Conjugated |
| Short name: |
FITC Conjugated, Ki-67 (Proliferation Marker) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Ki-67 (expantion detection labelled) (Antibody to) |
| Alternative technique: |
antibodies |