| Long name: |
Biotin Conjugated, Ki-67 (Proliferation Marker) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Ki-67 proliferation Marker |
| Specificity: |
This is a highly specific antibody against Ki-67 proliferation Marker |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1250-1300/3256 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human Ki67 |
| Gene ID number: |
4288 |
| Swiss Prot: |
P46013 |
| Tested applications: |
IHC-P |
| Recommended dilutions: |
IHC-P(1:100-500) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
G2 and M phase), In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index, It is absent in resting (G0) cells, Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index), Ki67 is routinely used as a neuronal marker of cell cycling and proliferation, S, The correlation between low Ki67 index and histologically low grade tumours is strong, expressed by proliferating cells in all phases of the active cell cycle (G1, Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
Antigen KI-67, MIB-, MIB-1, MKI67, PPP1R15, KIA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Ki-67 (Proliferation Conjugated |
| Short name: |
Conjugated, Ki-67 (Proliferation Marker) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Ki-67 (expantion detection labelled) (Antibody to) |
| Alternative technique: |
antibodies |