| Long name: |
ALEXA FLUOR 350 Conjugated, Yellow fever virus envelope glycoprotein E Antibody |
| Also known as: |
Anti-Yellow fever virus envelope glycoprotein E PAb ALEXA FLUOR 350 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
350, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Yellow fever virus envelope glycoprotein E |
| Specificity: |
This is a highly specific antibody against Yellow fever virus envelope glycoprotein E |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
650-700/3411 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from Yellow fever virus envelope glycoprotein E (IIVGRGDSRLTY) |
| Gene ID Number: |
1502173 |
| Swiss Prot: |
P03314 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Virus |
| Cross-reactive species details: |
Yellow fever virus |
| Background of the antigen: |
After cleavage by host furin, After cleavage of prM, Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis, Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes, NS2A-NS2B, NS2B-NS3, NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity), NS3 serine protease, NS3-NS4A, NS4A-2K and NS4B-NS5, NTPase and RNA helicase, Non-structural protein 1 is involved in virus replication and regulation of the innate immune response, Non-structural protein 2A may be involved viral RNA replication and capsid assembly, Non-structural protein 2B is a required cofactor for the serine protease function of NS3, Non-structural protein 4A induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, Serine protease NS3 displays three enzymatic activities: serine protease, Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E, The capsid encapsulates the genomic RNA (By similarity), envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity), functioning as a replication complex, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide, prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity), Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
343nm/442nm |
| Synonyms: |
Genome polyprotein |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 350, Alexa Fluor |
| Gene target: |
fever virus envelope glycoprotein E |
| Short name: |
fever virus envelope glycoprotein E Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 350 |
| Alternative name: |
ALEXA FLUOR 350, Yellow fever virus envelope glycoprotein E (Antibody to) |
| Alternative technique: |
antibodies |