| Long name: |
ALEXA FLUOR 647 Conjugated, c-Raf(Ser338/Tyr340) Polyclonal Antibody |
| Also known as: |
Anti-c-Raf Ser338/Tyr340 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
c-Raf Ser338/Tyr340 |
| Specificity: |
This is a highly specific antibody against c-Raf Ser338/Tyr340 |
| Modification: |
Phosphorylation |
| Modification Site: |
Ser338/Tyr340 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
310-360/648 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser338/Tyr340 |
| Gene ID Number: |
5894 |
| Swiss Prot: |
P04049 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
ADCY5 and ADCY6, Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death, Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity, Phosphorylates TNNT2/cardiac muscle troponin T, Phosphorylates adenylyl cyclases: ADCY2, Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2), Regulates Rho signaling and migration, Restricts caspase activation in response to selected stimuli, The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, and erythroid differentiation, and is required for normal wound healing, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, apoptosis, apoptosis (MAP3K5/ASK1 and STK3/MST2), by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75', differentiation, notably Fas stimulation, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, pathogen-mediated macrophage apoptosis, proliferation and angiogenesis (RB1), resulting in their activation, survival and oncogenic transformation, which induces down-regulation of OCLN, Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
CMD1NN, CRAF, Proto-oncogene c-RAF, RAF, RAF proto-oncogene serine/threonine-protein kinase, RAF1, Raf-1, c-Raf, NS5 |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the c-Raf(Ser338/Tyr340) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
c-Raf(Ser338/Tyr340) |
| Short name: |
c-Raf(Ser338/Tyr340) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, c-Raf(Ser338/Tyr340) (Antibody to) |
| Alternative technique: |
antibodies |