| Long name: |
FITC Conjugated, Hephaestin Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Hephaestin |
| Specificity: |
This is a highly specific antibody against Hephaestin |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Hephaestin |
| Gene ID number: |
9843 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Hephaestin, Hephaestin can bind six copper ions per monomer and is regulated by the homeobox transcription factor CDX2, Hephaestin is primarily detected in the intestine, Increased levels of iron leads to an increase in CDX2 expression and thus Hephaestin, It mediates the movement of iron across the basolateral membrane in conjunction with ferroportin 1, This is an important link between iron and copper metabolism in mammalian systems, a copper-dependant ferroxidase protein, as copper deficiency leads to reduced hephaestin and reduced iron absorption resulting in anemia, bone trabecural cells and fibroblasts, breast, but is also expressed in colon, is crucial for iron exiting intestinal enterocytes into the circulation, Hephaestin is a single-pass type I membrane protein that belongs to the multicopper oxidase family of proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HEPH, HEPH_HUMAN, Hephaestin, CPL |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Hephaestin Conjugated |
| Short name: |
FITC Conjugated, Hephaestin Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Hephaestin (Antibody to) |
| Alternative technique: |
antibodies |