| Long name: |
FITC Conjugated, ErbB-3/HER3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ErbB-3/HER3 |
| Specificity: |
This is a highly specific antibody against ErbB-3/HER3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
670-720/1342 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human ErbB-3 |
| Gene ID number: |
2065 |
| Swiss Prot: |
P21860 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Additional splice variants have also been reported but they have not been thoroughly characterized, Alternate transcriptional splice variants encoding different isoforms have been characterized, Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain, Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation, However it does form heterodimers with other EGF receptor family members which do have kinase activity, It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation, One isoform lacks the intermembrane region and is secreted outside the cell, This form acts to modulate the activity of the membrane-bound form, bladder and breast tumors, The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HER3 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ErbB-3/HER3 Conjugated |
| Short name: |
FITC Conjugated, ErbB-3/HER3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ErbB-3/HER3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3431 |
| Gene: |
ERBB3 |
More about : ERBB3 |
| Long gene name: |
erb-b2 receptor tyrosine kinase 3 |
| Synonyms gene: |
LCCS2 |
| Synonyms gene name: |
lethal congenital contracture syndrome 2 v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 |
| Synonyms name: |
human epidermal growth factor receptor 3 |
| Locus: |
12q13, 2 |
| Discovery year: |
1990-07-15 |
| GenBank acession: |
M34309 |
| Entrez gene record: |
2065 |
| RefSeq identity: |
NM_001982 |
| Classification: |
Erb-b2 receptor tyrosine kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000170140 |
| Locus Specific Databases: |
LRG_996 |