| Long name: |
FITC Conjugated, HIF2 alpha Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HIF2 alpha |
| Specificity: |
This is a highly specific antibody against HIF2 alpha |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1-50/874 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from mouse HIF2 Alpha |
| Gene ID number: |
13819 |
| Swiss Prot: |
P97481 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP3, Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters, Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity), May also play a role in the formation of the endothelium that gives rise to the blood brain barrier, Potent activator of the Tie-2 tyrosine kinase expression, Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung, Transcription factor involved in the induction of oxygen regulated genes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
EPAS-1, Endothelial PAS domain-containing protein 1, Epas1, HIF-1-alpha-like factor, HIF-2-alpha, HIF-2alpha, HIF-related factor, HIF2-alpha, HIF2A, HRF, Hypoxia-inducible factor 2-alpha, MOP2, bHLHe73, mHLF, HLF |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HIF2 alpha Conjugated |
| Short name: |
FITC Conjugated, HIF2 alpha Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HIF2 a (Antibody to) |
| Alternative technique: |
antibodies |