| Long name: |
FITC Conjugated, Adeno-Associated Virus Capsid Protein VP1 Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Adeno-Associated Virus Capsid Protein VP1 |
| Specificity: |
AAV3B, AAV4, AAV9, This antibody will recognize many AAV strains, and AAV13, including AAV5, This is a highly specific antibody against Adeno-Associated Virus Capsid Protein VP1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
30-80/724 |
| Source: |
KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Virus |
| Crossreactive species details: |
AAV5 |
| Antigen background: |
Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell, The capsid encapsulates the genomic ssDNA, This attachment induces virion internalization predominantly through clathrin-dependent endocytosis, VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus, VP2 and VP3 which differ in their N-terminus, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, specifically its phospholipase A2-like region and putative nuclear localization signal(s), Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Parvovirus coat protein VP1, capsid protein, capsid protein AAV5, capsid protein [Adeno-associated virus - 5], capsid protein Adeno-associated 5 virus |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Adeno-Associated Virus Capsid Protein VP1 Conjugated |
| Short name: |
FITC Conjugated, Adeno-Associated Virus Capsid Protein VP1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Species: |
Viruses, Virus |
| Alternative name: |
fluorecein coupled, Adeno-Associated Virus Capsid Protein VP1 (Antibody to) |
| Alternative technique: |
antibodies |