| Long name: |
Biotin Conjugated, Adeno-Associated Virus Capsid Protein VP1 Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Adeno-Associated Virus Capsid Protein VP1 |
| Specificity: |
AAV3B, AAV4, AAV9, This antibody will recognize many AAV strains, and AAV13, including AAV5, This is a highly specific antibody against Adeno-Associated Virus Capsid Protein VP1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
30-80/724 |
| Source: |
KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Virus |
| Crossreactive species details: |
AAV5 |
| Antigen background: |
Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell, The capsid encapsulates the genomic ssDNA, This attachment induces virion internalization predominantly through clathrin-dependent endocytosis, VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus, VP2 and VP3 which differ in their N-terminus, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, specifically its phospholipase A2-like region and putative nuclear localization signal(s), Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
Parvovirus coat protein VP1, capsid protein, capsid protein AAV5, capsid protein [Adeno-associated virus - 5], capsid protein Adeno-associated 5 virus |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Adeno-Associated Virus Capsid Protein VP1 Conjugated |
| Short name: |
Conjugated, Adeno-Associated Virus Capsid Protein VP1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Species: |
Viruses, Virus |
| Alternative name: |
biotinilated coupled, Adeno-Associated Virus Capsid Protein VP1 (Antibody to) |
| Alternative technique: |
antibodies |