| Antigen detectable with this ELISA kit: |
Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
116ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1), The concentration of Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) |
| Short name: |
Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Species: |
Viruses, Virus |
| Alternative name: |
Spleen Focus Forming Virus Proviral Integration Oncogene (spleen focus forming virus (SFFV) proviral integration oncogene spi1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0030098 and lymphocyte differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030225 and macrophage differentiation and biological process this GO :0030851 and granulocyte differentiation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0043011 and myeloid dendritic cell differentiation and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0043966 and histone H3 acetylation and biological process this GO :0044027 and hypermethylation of CpG island and biological process this GO :0045087 and innate immune response and biological process this GO :0045347 and negative regulation of MHC class II biosynthetic process and biological process this GO :0045646 and regulation of erythrocyte differentiation and biological process this GO :0045814 and negative regulation of gene expression, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051525 and NFAT protein binding and molecular function this GO :0060033 and anatomical structure regression and biological process this GO :0090241 and negative regulation of histone H4 acetylation and biological process this GO :1902262 and apoptotic process involved in patterning of blood vessels and biological process, NFAT protein binding, OF and PU, SPI1 and IDBG-43260 and ENSG00000066336 and 6688, Sfpi1 and IDBG-190185 and ENSMUSG00000002111 and 20375, epigenetic and biological process this GO :0045892 and negative regulation of transcription, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000979 and RNA polymerase II core promoter sequence-specific DNA binding and molecular function this GO :0000980 and RNA polymerase II distal enhancer sequence-specific DNA binding and molecular function this GO :0000981 and sequence-specific DNA binding RNA polymerase II transcription factor activity and molecular function this GO :0001047 and core promoter binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001085 and RNA polymerase II transcription factor binding and molecular function this GO :0001205 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001944 and vasculature development and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0003723 and RNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006355 and regulation of transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding and also this GO :0000980 : RNA polymerase II distal enhancer sequence-specific DNA binding and also this GO :0000981 : sequence-specific DNA binding RNA polymerase II transcription factor activity and also this GO :0001047 : core promoter binding and also this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0001085 : RNA polymerase II transcription factor binding and also this GO :0001205 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0003723 : RNA binding and also this GO :0005515 : protein binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0051525 : NFAT protein binding, this GO :0000979 : RNA polymerase II core promoter sequence-specific DNA binding, this GO :0000980 : RNA polymerase II distal enhancer sequence-specific DNA binding, this GO :0000981 : sequence-specific DNA binding RNA polymerase II transcription factor activity, this GO :0001047 : core promoter binding, this GO :0001077 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0001085 : RNA polymerase II transcription factor binding, this GO :0001205 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0003723 : RNA binding, this GO :0005515 : protein binding, this GO :0043565 : sequence-specific DNA binding, this GO :0051525 : NFAT protein binding, 1 and SFPI1 and SPI-1 and SPI-A, 58934 and IDBG-637435 and ENSBTAG00000021709 and 507100, spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
| Identity: |
11241 |
| Gene: |
SPI1 |
More about : SPI1 |
| Long gene name: |
Spi-1 proto-oncogene |
| Synonyms gene name: |
spleen focus forming virus (SFFV) proviral integration oncogene |
| Synonyms: |
PU, 1 SPI-A OF SFPI1 SPI-1 |
| Synonyms name: |
hematopoietic transcription factor PU, 1 31 kDa transforming protein |
| Locus: |
11p11, 2 |
| Discovery year: |
1988-07-04 |
| GenBank acession: |
X52056 |
| Entrez gene record: |
6688 |
| Pubmed identfication: |
1693183 |
| RefSeq identity: |
NM_003120 |
| Classification: |
ETS transcription factor family |
| Havana BLAST/BLAT: |
OTTHUMG00000150150 |