| Also known as: |
Bromodeoxyuridine (Brdu) |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Rat IgG2a |
| Clone: |
BU1/75 (ICR1) |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, N/A |
| Specificity and cross-reactivity: |
BrdU attached to a protein carrier or free BrdU, It also reacts with chlorodeoxyuridine but with reduced staining, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA, The antibody does not cross react with thymidine), This particular antibody might cross react with speacies outside of the listed ones, Bromodeoxyuridine (Brdu) (Reacts with BrdU in single stranded DNA |
| Purification method: |
Protein G |
| Form/Appearance: |
Biotin (Purified Ig in PBS/Glycerol (50%) + BSA (10 mg/ml)) |
| Storage and shipping: |
Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Storage in frost-free freezers is not recommended, for longer periods, for one month, or in small aliquots the antibody should be stored at -20 degrees Celsius, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunocytochemistry (ICC) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, Biotin conjugates can be detected by horseradish peroxidase |
| Conjugation: |
Biotinylated |
| About: |
Rats , There are less rat- than mouse clones however, Rats are used to make rat monoclonal anti mouse antibodies, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Latin name: |
Rattus norvegicus |
| Gene target: |
BU1/75 (ICR1) IgG2a), Bromodeoxyuridine (Brdu) |
| Short name: |
(Clone: BU1/75 (ICR1)) ( IgG2a), Anti-Bromodeoxyuridine (Brdu) |
| Host: |
Rat |
| Isotype: |
IgG2a |
| Label: |
Biotin |
| Species: |
Rats, Rat |
| Alternative name: |
(Rat IgG2a), (clonality: BU1/75 (ICR1)), biotinilated, antibody to-Bromodeoxyuridine (Bromodeoxyuridine) |
| Alternative technique: |
immunoglobulins |
| Identity: |
5352 |
| Gene: |
ICR1 |
More about : ICR1 |
| Long gene name: |
erythromatous lamellar ichthyosis, ichthyosiform erythroderma , ichthyosis congenita I |
| Locus: |
reserved |
| Discovery year: |
1991-07-04 |