| Also known as: |
Bromodeoxyuridine (Brdu) |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Rat IgG2a |
| Clone: |
BU1/75 (ICR1) |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, N/A |
| Specificity and cross-reactivity: |
BrdU attached to a protein carrier or free BrdU, It also reacts with chlorodeoxyuridine but with reduced staining, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA, The antibody does not cross react with thymidine), This particular antibody might cross react with speacies outside of the listed ones, Bromodeoxyuridine (Brdu) (Reacts with BrdU in single stranded DNA |
| Purification method: |
Protein G |
| Form/Appearance: |
0, 50% Glycerol, 1% Sodium Azide), FITC (Purified Ig in PBS |
| Storage and shipping: |
Avoid repeated freezing and thawing as this may denature the antibody, Avoid repeated freezing and thawing as this may denature the antibodyStore the antibody at +4 degrees Celsius, Storage in frost-free freezers is not recommended, Storage in frost-free freezers is not recommended, for longer periods, for longer periods, for one month, for one month, or in small aliquots the antibody should be stored at -20 degrees Celsius, or in small aliquots the antibody should be stored at -20 degrees Celsius, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunocytochemistry (ICC) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Rats , There are less rat- than mouse clones however, Rats are used to make rat monoclonal anti mouse antibodies, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Latin name: |
Rattus norvegicus |
| Gene target: |
BU1/75 (ICR1) IgG2a), Bromodeoxyuridine (Brdu) |
| Short name: |
(Clone: BU1/75 (ICR1)) ( IgG2a), Anti-Bromodeoxyuridine (Brdu) FITC |
| Technique: |
Fluorescein, FITC |
| Host: |
Rat |
| Isotype: |
IgG2a |
| Label: |
FITC |
| Species: |
Rats, Rat |
| Alternative name: |
(Rat IgG2a), (clonality: BU1/75 (ICR1)), fluorecein, antibody to-Bromodeoxyuridine (Bromodeoxyuridine) |
| Alternative technique: |
fluorescine |
| Identity: |
5352 |
| Gene: |
ICR1 |
More about : ICR1 |
| Long gene name: |
erythromatous lamellar ichthyosis, ichthyosiform erythroderma , ichthyosis congenita I |
| Locus: |
reserved |
| Discovery year: |
1991-07-04 |