| Also known as: |
FTO |
| Other names: |
AlkB homolog 9, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, alpha-ketoglutarate-dependent dioxygenase FTO, fat mass and obesity associated, fat mass and obesity-associated protein, protein fto, alpha-ketoglutarate-dependent dioxygenase FTO |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
FTO |
| Other gene names: |
ALKBH9, FTO, KIAA1752, FTO |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG |
| Clone: |
5-2H10 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Dog, Due to limited knowledge and inability for testing each and every species, Horse, Human (Homo sapiens), Mouse (Mus musculus), Primate, Rat (Rattus norvegicus), Sheep, the reactivity of the antibody may extend to other species which are not listed hereby, Bovine |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The antibody has also been used for immunocytochemistry with neuronal progenitor cells, This particular antibody might cross react with speacies outside of the listed ones, Specific for the ~58 kDa Fto protein in Western blots of rat testes lysate |
| Purification method: |
Protein G Purified |
| Form/Appearance: |
100 ug BSA per ml and 50% glycerol, 150 mM NaCl, 10 mM HEPES (pH 7, 5) |
| Storage and shipping: |
For long term storage aliquot and keep the antibody at -20 degrees Celsius, Freeze-thaw cycles should be avoided in order to retain the activity of the antibody, Store undiluted, Store at +4 degrees Celsius for short term storage |
| Tested applications:: |
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC - Paraffin) |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| About: |
2 light chains, Each murine monoclonal anibody has his own affinity specific for the clone, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, Monoclonals of this antigen are available in different clones, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Rats are used to make rat monoclonal anti mouse antibodies, Rats , The IgG antibody has 2 , There are 2 heavy chains, There are less rat- than mouse clones however, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, antibodies, antigen , binding sites, genes from rodents , have 4 parts, of the genus , serum |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Rattus norvegicus, Mus musculus |
| Gene target: |
Monoclonal [ 5-2H10] FTO |
| Short name: |
Mouse Monoclonal [clone 5-2H10] (IgG) FTO Antibody |
| Technique: |
IgGs, Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Host: |
Rat |
| Isotype: |
IgG |
| Species: |
Mouses, Rats, Rat |
| Alternative name: |
Mouse monoclonal [clonality 5-2H10] (Immunoglobulin G) to Rat fat mass and obesity associated (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-N1-methyladenine dioxygenase activity, FTO and IDBG-31147 and ENSG00000140718 and 79068, FTO and IDBG-631892 and ENSBTAG00000012501 and 618622, Fto and IDBG-179938 and ENSMUSG00000055932 and 26383, nuclei, this GO :0001659 and temperature homeostasis and biological process this GO :0005634 and nucleus and cellular component this GO :0006307 and DNA dealkylation involved in DNA repair and biological process this GO :0008198 and ferrous iron binding and molecular function this GO :0010883 and regulation of lipid storage and biological process this GO :0016607 and nuclear speck and cellular component this GO :0035515 and oxidative RNA demethylase activity and molecular function this GO :0035516 and oxidative DNA demethylase activity and molecular function this GO :0035552 and oxidative single-stranded DNA demethylation and biological process this GO :0035553 and oxidative single-stranded RNA demethylation and biological process this GO :0040014 and regulation of multicellular organism growth and biological process this GO :0042245 and RNA repair and biological process this GO :0043734 and DNA-N1-methyladenine dioxygenase activity and molecular function this GO :0044065 and regulation of respiratory system process and biological process this GO :0060612 and adipose tissue development and biological process this GO :0070350 and regulation of white fat cell proliferation and biological process this GO :0070989 and oxidative demethylation and biological process this GO :0080111 and DNA demethylation and biological process, this GO :0008198 : ferrous iron binding, this GO :0008198 : ferrous iron binding and also this GO :0035515 : oxidative RNA demethylase activity and also this GO :0035516 : oxidative DNA demethylase activity and also this GO :0043734 : DNA-N1-methyladenine dioxygenase activity, this GO :0035515 : oxidative RNA demethylase activity, this GO :0035516 : oxidative DNA demethylase activity, this GO :0043734 : DNA-N1-methyladenine dioxygenase activity, fat mass and obesity associated |
| Identity: |
24678 |
| Gene: |
FTO |
More about : FTO |
| Long gene name: |
FTO, alpha-ketoglutarate dependent dioxygenase |
| Synonyms gene name: |
fat mass and obesity associated |
| Synonyms: |
KIAA1752 MGC5149 ALKBH9 |
| Synonyms name: |
alkB homolog 9 alpha-ketoglutarate-dependent dioxygenase |
| Locus: |
16q12, 2 |
| Discovery year: |
2007-04-12 |
| GenBank acession: |
BC003583 |
| Entrez gene record: |
79068 |
| Pubmed identfication: |
17434869 17991826 22002720 |
| RefSeq identity: |
NM_001080432 |
| Classification: |
Alkylation repair homologs |
| Havana BLAST/BLAT: |
OTTHUMG00000158780 |