| Reacts with: |
Mouse |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Mouse Semaphorin 4A is produced by our Mammalian expression system and the target gene encoding Thr33-His682 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
7 kD, 72 |
| UniProt number: |
Q62178 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
150 mM sodium chloride, 2 um filtered solution of 20 mM PB, 4, Lyophilized from a 0, pH7 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
TGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPHVDHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Group: |
recombinants |
| Gene target: |
SEMA4A (C-6His), Semaphorin-4A |
| Short name: |
SEMA4A (C-6His), Recombinant Mouse Semaphorin-4A |
| Technique: |
E, coli recombinant proteins are , mouses, novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Mouses, Mouse |
| Alternative name: |
(semaphorin) 4A (C-6His), immunoglobulin domain (Ig), sema domain, transmembrane domain (TM) and short cytoplasmic domain, recombinant Mouse Semaphorin-4A |
| Alternative technique: |
rec |
| Alternative to gene target: |
(semaphorin) 4A, Plasma membranes, SEMA4A and IDBG-103399 and ENSG00000196189 and 64218, SEMA4A and IDBG-631665 and ENSBTAG00000012228 and 510239, Sema4a and IDBG-160873 and ENSMUSG00000028064 and 20351, immunoglobulin domain (Ig), protein binding, this GO :0001525 and angiogenesis and biological process this GO :0002292 and T cell differentiation involved in immune response and biological process this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007275 and multicellular organismal development and biological process this GO :0007409 and axonogenesis and biological process this GO :0007411 and axon guidance and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0010594 and regulation of endothelial cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0045063 and T-helper 1 cell differentiation and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10729 |
| Gene: |
SEMA4A |
More about : SEMA4A |
| Long gene name: |
semaphorin 4A |
| Synonyms gene: |
SEMAB |
| Synonyms gene name: |
(semaphorin) 4A , immunoglobulin domain (Ig), sema domain, transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms: |
SemB FLJ12287 CORD10 |
| Locus: |
1q22 |
| Discovery year: |
2004-04-22 |
| GenBank acession: |
AK022416 |
| Entrez gene record: |
64218 |
| Pubmed identfication: |
7748561 |
| RefSeq identity: |
NM_022367 |
| Classification: |
Semaphorins |
| Havana BLAST/BLAT: |
OTTHUMG00000014042 |