| Antigen detectable with this ELISA kit: |
Semaphorin 4A (SEMA4A) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
131ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4A (SEMA4A), The concentration of Semaphorin 4A (SEMA4A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4A (SEMA4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Semaphorin 4A (SEMA4A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Semaphorin 4A (SEMA4A) |
| Short name: |
Semaphorin 4A (SEMA4A) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
(semaphorin) 4A) Enzyme-linked immunosorbent assay reagent, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, Semaphorin 4A (sema domain |
| Alternative technique: |
elisas |
| Alternative to gene target: |
(semaphorin) 4A, Plasma membranes, SEMA4A and IDBG-103399 and ENSG00000196189 and 64218, SEMA4A and IDBG-631665 and ENSBTAG00000012228 and 510239, Sema4a and IDBG-160873 and ENSMUSG00000028064 and 20351, immunoglobulin domain (Ig), protein binding, this GO :0001525 and angiogenesis and biological process this GO :0002292 and T cell differentiation involved in immune response and biological process this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007275 and multicellular organismal development and biological process this GO :0007409 and axonogenesis and biological process this GO :0007411 and axon guidance and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0010594 and regulation of endothelial cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0045063 and T-helper 1 cell differentiation and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity: |
10729 |
| Gene: |
SEMA4A |
More about : SEMA4A |
| Long gene name: |
semaphorin 4A |
| Synonyms gene: |
SEMAB |
| Synonyms gene name: |
(semaphorin) 4A , immunoglobulin domain (Ig), sema domain, transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms: |
SemB FLJ12287 CORD10 |
| Locus: |
1q22 |
| Discovery year: |
2004-04-22 |
| GenBank acession: |
AK022416 |
| Entrez gene record: |
64218 |
| Pubmed identfication: |
7748561 |
| RefSeq identity: |
NM_022367 |
| Classification: |
Semaphorins |
| Havana BLAST/BLAT: |
OTTHUMG00000014042 |