| Reacts with: |
Human |
| Source: |
proteins, Recombinants or rec |
| Description: |
Recombinant Human Semaphorin 3A is produced by our Mammalian expression system and the target gene encoding Asn21-Val771 is expressed with a 6His tag at the C-terminus |
| Molecular Weight: |
3 kD, 87 |
| UniProt number: |
Q14563 |
| State of the product: |
Freeze-dried |
| Shipping conditions: |
Ambient temperature |
| Formulation: |
pH 7, 2 um filtered solution of phosphate buffered saline, 4, Lyophilized from a 0 |
| Storage recommendations: |
-20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution: |
Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity: |
and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid: |
NYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRATRAQDIRNGDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRTDQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQKVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRSVHHHHHH |
| Levels of endotoxin: |
11 IEU/ug, LAL test shows less than than 0 |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group: |
recombinants |
| Gene target: |
SEMA3A (C-6His), Semaphorin-3A |
| Short name: |
SEMA3A (C-6His), Recombinant Semaphorin-3A |
| Technique: |
E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species: |
Humans, Human |
| Alternative name: |
(semaphorin) 3A (C-6His), immunoglobulin domain (Ig), sapiens Semaphorin-3A, secreted, sema domain, short basic domain, recombinant H |
| Alternative technique: |
rec |
| Alternative to gene target: |
(semaphorin) 3A, Extracellular, SEMA3A and IDBG-24546 and ENSG00000075213 and 10371, SEMA3A and IDBG-630984 and ENSBTAG00000018133 and 537777, Sema3a and IDBG-135492 and ENSMUSG00000028883 and 20346, chemorepellent activity, coll-1 and COLL1 and HH16 and Hsema-I and Hsema-III and SEMA1 and SEMAD and SEMAIII and SEMAL and SemD, immunoglobulin domain (Ig), secreted, short basic domain, this GO :0001764 and neuron migration and biological process this GO :0002027 and regulation of heart rate and biological process this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0006915 and apoptotic process and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007411 and axon guidance and biological process this GO :0007413 and axonal fasciculation and biological process this GO :0008045 and motor neuron axon guidance and biological process this GO :0010633 and negative regulation of epithelial cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0021612 and facial nerve structural organization and biological process this GO :0021637 and trigeminal nerve structural organization and biological process this GO :0021675 and nerve development and biological process this GO :0021772 and olfactory bulb development and biological process this GO :0021785 and branchiomotor neuron axon guidance and biological process this GO :0021828 and this GO nadotrophin-releasing hormone neuronal migration to the hypothalamus and biological process this GO :0030215 and semaphorin receptor binding and molecular function this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0030517 and negative regulation of axon extension and biological process this GO :0038191 and neuropilin binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045499 and chemorepellent activity and molecular function this GO :0048485 and sympathetic nervous system development and biological process this GO :0048813 and dendrite morphogenesis and biological process this GO :0048841 and regulation of axon extension involved in axon guidance and biological process this GO :0048843 and negative regulation of axon extension involved in axon guidance and biological process this GO :0048846 and axon extension involved in axon guidance and biological process this GO :0048880 and sensory system development and biological process this GO :0050919 and negative chemotaxis and biological process this GO :0060385 and axonogenesis involved in innervation and biological process this GO :0060666 and dichotomous subdivision of terminal units involved in salivary gland branching and biological process this GO :0061549 and sympathetic ganglion development and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process this GO :0097490 and sympathetic neuron projection extension and biological process this GO :0097491 and sympathetic neuron projection guidance and biological process this GO :1901166 and neural crest cell migration involved in autonomic nervous system development and biological process this GO :1902285 and semaphorin-plexin signaling pathway involved in neuron projection guidance and biological process this GO :1902287 and semaphorin-plexin signaling pathway involved in axon guidance and biological process this GO :1903045 and neural crest cell migration involved in sympathetic nervous system development and biological process this GO :2000020 and positive regulation of male this GO nad development and biological process this GO :2001224 and positive regulation of neuron migration and biological process, this GO :0004872 : receptor activity, this GO :0004872 : receptor activity and also this GO :0005515 : protein binding and also this GO :0030215 : semaphorin receptor binding and also this GO :0038191 : neuropilin binding and also this GO :0045499 : chemorepellent activity, this GO :0005515 : protein binding, this GO :0030215 : semaphorin receptor binding, this GO :0038191 : neuropilin binding, this GO :0045499 : chemorepellent activity, sema domain |
| Identity: |
10723 |
| Gene: |
SEMA3A |
More about : SEMA3A |
| Long gene name: |
semaphorin 3A |
| Synonyms gene: |
SEMAD |
| Synonyms gene name: |
(semaphorin) 3A , immunoglobulin domain (Ig), secreted, sema domain, short basic domain |
| Synonyms: |
SEMA1 SemD coll-1 Hsema-I |
| Synonyms name: |
sema III |
| Locus: |
7q21, 11 |
| Discovery year: |
1999-06-25 |
| GenBank acession: |
L26081 |
| Entrez gene record: |
10371 |
| Pubmed identfication: |
8269517 7748561 |
| RefSeq identity: |
NM_006080 |
| Classification: |
Semaphorins Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000023443 |