| Also known as: |
I-Ab and I-Ad |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Mouse IgG2a |
| Clone: |
25-9-17S |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, I-Ab, d (Mouse I-Ab) |
| Form/Appearance: |
(Purified from ascitic fluid via Protein G Chromatography)), 0, 02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml, FITC (FITC conjugated Ig buffered in PBS |
| Concentration: |
1 miligram per 1 mililiter, Antibody Concentration: 0 |
| Storage and shipping: |
Avoid freeze/thaw cycles, Avoid prolonged exposure to light, For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius, in volumes appropriate for single usage, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunohistochemistry (IHC) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
25-9-17S) IgG2a), I-Ab I-Ad |
| Short name: |
FITC (Clone 25-9-17S) (mouse IgG2a), Anti-Mouse I-Ab I-Ad |
| Technique: |
Fluorescein, mouses, Mouse |
| Host: |
mouse |
| Isotype: |
IgG2a |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
fluorecein (clonality 25-9-17S) (mouse IgG2a), antibody to-Mouse I-Antibody and I-Ad |
| Alternative technique: |
murine |