| Also known as: |
I-Ab and I-Ad |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Mouse IgG2a |
| Clone: |
25-9-17S |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, I-Ab, d (Mouse I-Ab) |
| Form/Appearance: |
(Purified from ascitic fluid via Protein G Chromatography)), 0, 02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml, Biotin (Biotin conjugated Ig buffered in PBS |
| Concentration: |
1 miligram per 1 mililiter, Antibody Concentration: 0 |
| Storage and shipping: |
Avoid freeze/thaw cycles, For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius, in volumes appropriate for single usage, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunohistochemistry (IHC) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, Biotin conjugates can be detected by horseradish peroxidase |
| Conjugation: |
Biotinylated |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
25-9-17S) IgG2a), I-Ab I-Ad |
| Short name: |
(Clone 25-9-17S) (mouse IgG2a), Anti-Mouse I-Ab I-Ad |
| Technique: |
mouses, Mouse |
| Host: |
mouse |
| Isotype: |
IgG2a |
| Label: |
Biotin |
| Species: |
Mouses, Mouse |
| Alternative name: |
biotinilated (clonality 25-9-17S) (mouse IgG2a), antibody to-Mouse I-Antibody and I-Ad |
| Alternative technique: |
murine |