| Long name: |
Biotin Conjugated, GIT2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GIT2 |
| Specificity: |
This is a highly specific antibody against GIT2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human GIT2 |
| Gene ID number: |
9815 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3, thus reducing b2-adrenergic receptor signaling (5), which are involved in vesicular trafficking (5, 2), 4), 6), Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
KIAA0148 PKL |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GIT2 Conjugated |
| Short name: |
Conjugated, GIT2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, GIT2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4273 |
| Gene: |
GIT2 |
More about : GIT2 |
| Long gene name: |
GIT ArfGAP 2 |
| Synonyms gene name: |
G protein-coupled receptor kinase interactor 2 G protein-coupled receptor kinase interacting ArfGAP 2 |
| Synonyms name: |
Paxillin kinase linker |
| Locus: |
12q24, 11 |
| Discovery year: |
2000-07-31 |
| GenBank acession: |
AF124491 |
| Entrez gene record: |
9815 |
| Pubmed identfication: |
9826657 10896954 |
| RefSeq identity: |
NM_057169 |
| Classification: |
Ankyrin repeat domain containing ArfGAPs |
| Havana BLAST/BLAT: |
OTTHUMG00000169313 |