| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GIT2 |
| Specificity: |
This antibody reacts specifically with GIT2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GIT2 |
| Gene ID: |
9815 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3, thus reducing b2-adrenergic receptor signaling (5), which are involved in vesicular trafficking (5, 2), 4), 6), Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA0148 PKL |
| Also known as: |
GIT2 Polyclonal Antibody |
| Other name: |
Anti-GIT2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GIT2 |
| Short name: |
Anti-GIT2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GIT2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4273 |
| Gene: |
GIT2 |
More about : GIT2 |
| Long gene name: |
GIT ArfGAP 2 |
| Synonyms gene name: |
G protein-coupled receptor kinase interactor 2 G protein-coupled receptor kinase interacting ArfGAP 2 |
| Synonyms name: |
Paxillin kinase linker |
| Locus: |
12q24, 11 |
| Discovery year: |
2000-07-31 |
| GenBank acession: |
AF124491 |
| Entrez gene record: |
9815 |
| Pubmed identfication: |
9826657 10896954 |
| RefSeq identity: |
NM_057169 |
| Classification: |
Ankyrin repeat domain containing ArfGAPs |
| Havana BLAST/BLAT: |
OTTHUMG00000169313 |