| Long name: |
ALEXA FLUOR 594 Conjugated, GAD67 Polyclonal Antibody |
| Also known as: |
Anti-GAD67/GAD1 PAb ALEXA FLUOR 594 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GAD67/GAD1 |
| Specificity: |
This is a highly specific antibody against GAD67/GAD1 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
510-560/594 |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human GAD67 |
| Gene ID Number: |
2571 |
| Swiss Prot: |
Q99259 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
(585 amino acid residues) and is encoded on human chromosome 10, GAD exists as two isoforms, GAD has a restricted tissue distribution, GAD is also transiently expressed in non-GABAergic cells of the embryonic and adult nervous system, GAD65 and GAD67 (molecular masses of 65 and 67 kD, GAD65 has been identified as an autoantigen in insulindependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS), GAD65 is an ampiphilic, GAD67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2, IDDM is an autoimmune disease that results from T cell mediated destruction of pancreatic insulin-secreting beta cells, Islet-reactive T cells and primarily to GAD65 (also named beta cell autoantigen) can be detected in peripheral blood of 80% of recent-onset IDD patients and in pre-diabetic high-risk subjects before onset of clinical symptoms, It is also present in other non-neuronal tissues such as testis, It is highly expressed in the cytoplasm of GABAergic neurons in the central nervous system (CNS) and pancreatic beta cells, The two isoforms appear to have distinct intraneuronal distribution in the brain, There is 64% amino acid identity between the two isoforms, This suggests that GAD may be an important marker in the early stages of the disease, and a putative paracrine signal molecule in pancreatic islets, membraneanchored protein, oviduct and ovary, respectively) that are encoded by two different genes, suggesting its involvement in development and plasticity, the principal inhibitory neurotransmitter in the brain, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles, with the highest diversity located at the N terminus, Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L glutamate to g-aminobutyric acid (GABA) |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
67 kDa glutamic acid decarboxylase, CPSQ1, GAD-67, GAD1, GAD67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform, SCP, GAD |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
594, ALEXA FLUOR®, Alexa Fluor |
| Conjugated: |
Alexa conjugate 1 |
| Gene target: |
GAD67 |
| Short name: |
GAD67 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 594 |
| Alternative name: |
ALEXA FLUOR 594, GAD67 (Antibody to) |
| Alternative technique: |
antibodies |