| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GAD65 + GAD67 |
| Specificity: |
This antibody reacts specifically with GAD65 + GAD67 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GAD65 |
| Gene ID: |
2571, 2572 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes, The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid, This gene may also play a role in the stiff man syndrome, identified as a major autoantigen in insulin-dependent diabetes, This gene encodes one of several forms of glutamic acid decarboxylase |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
GAD65 |
| Also known as: |
GAD65 + GAD67 Polyclonal Antibody |
| Other name: |
Anti-GAD65 + GAD67 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GAD65 + GAD67 |
| Short name: |
Anti-GAD65 + GAD67 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GAD65 and GAD67 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4093 |
| Gene: |
GAD2 |
More about : GAD2 |
| Long gene name: |
glutamate decarboxylase 2 |
| Synonyms gene name: |
65kD) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 (pancreatic islets and brain |
| Locus: |
10p12, 1 |
| Discovery year: |
1991-11-29 |
| GenBank acession: |
AJ251501 |
| Entrez gene record: |
2572 |
| Pubmed identfication: |
2039509 |
| RefSeq identity: |
NM_000818 |
| Havana BLAST/BLAT: |
OTTHUMG00000017836 |