| Long name: |
Biotin Conjugated, ATP5E Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ATP5E |
| Specificity: |
This is a highly specific antibody against ATP5E |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ATP5E |
| Gene ID number: |
514 |
| Tested applications: |
IHC-P |
| Recommended dilutions: |
IHC-P(1:100-500) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ATPases contain two linked complexes: F1, F0 consists of three subunits: a, F1 consists of three Alpha chains and three Beta chains, Mitochondrial ATPase e chain (ATP5E) localizes to the mitochondria and catalyzes ATP synthesis, The e chain of F1 contains 50 amino acids and is the smallest of the five ATPase F1 chains, and F0, as well as one Gamma chain, b and c, one Delta chain and one e chain, the hydrophilic catalytic core, the membrane-embedded protein channel, which are weakly homologous, Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ATP5E Conjugated |
| Short name: |
Conjugated, ATP5E Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, ATP5E (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
838 |
| Gene: |
ATP5E |
More about : ATP5E |
| Long gene name: |
ATP synthase, H+ transporting, epsilon subunit , mitochondrial F1 complex |
| Locus: |
20q13, 32 |
| Discovery year: |
1993-02-25 |
| GenBank acession: |
AF077045 |
| Entrez gene record: |
514 |
| Pubmed identfication: |
10727396 |
| RefSeq identity: |
NM_001001977 |
| Classification: |
F-type ATPases Mitochondrial complex V: ATP synthase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000032854 |