| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ATP5E |
| Specificity: |
This antibody reacts specifically with ATP5E |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human ATP5E |
| Gene ID: |
514 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ATPases contain two linked complexes: F1, F0 consists of three subunits: a, F1 consists of three Alpha chains and three Beta chains, Mitochondrial ATPase e chain (ATP5E) localizes to the mitochondria and catalyzes ATP synthesis, The e chain of F1 contains 50 amino acids and is the smallest of the five ATPase F1 chains, and F0, as well as one Gamma chain, b and c, one Delta chain and one e chain, the hydrophilic catalytic core, the membrane-embedded protein channel, which are weakly homologous, Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
ATP5E Polyclonal Antibody |
| Other name: |
Anti-ATP5E Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ATP5E |
| Short name: |
Anti-ATP5E (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ATP5E (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
838 |
| Gene: |
ATP5E |
More about : ATP5E |
| Long gene name: |
ATP synthase, H+ transporting, epsilon subunit , mitochondrial F1 complex |
| Locus: |
20q13, 32 |
| Discovery year: |
1993-02-25 |
| GenBank acession: |
AF077045 |
| Entrez gene record: |
514 |
| Pubmed identfication: |
10727396 |
| RefSeq identity: |
NM_001001977 |
| Classification: |
F-type ATPases Mitochondrial complex V: ATP synthase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000032854 |