| Long name: |
Biotin Conjugated, CPA3/MC-CPA Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CPA3/MC-CPA |
| Specificity: |
This is a highly specific antibody against CPA3/MC-CPA |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human CPA3/MC-CPA |
| Gene ID number: |
1359 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
CPA3, MC-CPA is stored as an active enzyme in the granule and is released, MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues, Mast cell carboxypeptidase A (MC-CPA), The MC-CPA gene, The optimum pH of MC-CPA is between neutral and basic, a part of the peptidase M14 family, along with other inflammatory mediators, along with trytases and chymases, depending upon the substrate, is a highly conserved metalloprotease localized to the secretory granules, resides on chromosome 3 and contains 11 exons, upon mast cell degranulation, Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CPA3/MC-CPA Conjugated |
| Short name: |
Conjugated, CPA3/MC-CPA Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, carboxypeptidase A3 (mast cell)/MC-CPA (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CPA3 and IDBG-60396 and ENSG00000163751 and 1359, CPA3 and IDBG-637892 and ENSBTAG00000015008 and 528454, Cpa3 and IDBG-133537 and ENSMUSG00000001865 and 12873, Extracellular, MC-CPA, this GO :0002002 and regulation of angiotensin levels in blood and biological process this GO :0002003 and angiotensin maturation and biological process this GO :0004180 and carboxypeptidase activity and molecular function this GO :0004181 and metallocarboxypeptidase activity and molecular function this GO :0005576 and extracellular region and cellular component this GO :0006508 and proteolysis and biological process this GO :0008233 and peptidase activity and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0030133 and transport vesicle and cellular component this GO :0030141 and secretory granule and cellular component this GO :0044267 and cellular protein metabolic process and biological process, this GO :0004180 : carboxypeptidase activity, this GO :0004180 : carboxypeptidase activity and also this GO :0004181 : metallocarboxypeptidase activity and also this GO :0008233 : peptidase activity and also this GO :0008270 : zinc ion binding, this GO :0004181 : metallocarboxypeptidase activity, this GO :0008233 : peptidase activity, this GO :0008270 : zinc ion binding, zinc ion binding, carboxypeptidase A3 (mast cell) |
| Identity: |
2298 |
| Gene: |
CPA3 |
More about : CPA3 |
| Long gene name: |
carboxypeptidase A3 |
| Synonyms gene name: |
carboxypeptidase A3 (mast cell) |
| Synonyms name: |
mast cell carboxypeptidase A tissue carboxypeptidase A |
| Locus: |
3q24 |
| Discovery year: |
1990-06-13 |
| Entrez gene record: |
1359 |
| RefSeq identity: |
NM_001870 |
| Classification: |
Carboxypeptidases |
| Havana BLAST/BLAT: |
OTTHUMG00000159526 |