| Long name: |
FITC Conjugated, CYB5R3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CYB5R3 |
| Specificity: |
This is a highly specific antibody against CYB5R3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human CYB5R3 |
| Gene ID number: |
1727 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A serine residue at position 117 seems to only be found in persons of African origin, Asians, CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms, CYB5R3 is involved in the desaturation and elongation of fatty acids, Defects in CYB5R3 are the cause of hereditary methemoglobinemia (HM), Indo-Aryans or Arabs, Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, It is not found in Caucasians, The allele frequency is 0, There are three forms of this disease: type 1 (HM1), This difference seems to have no effect on the enzyme activity, Type 2 is a severe form accompanied by mental retardation and neurological impairment, and type 3 (HM3), cholesterol biosynthesis, drug metabolism and, in erythrocytes, in which the enzyme is completely deficient, in which the enzyme is only deficient in erythrocytes with a mild cyanosis, methemoglobin reduction, type 2 (HM2), where the deficiency is seen in all blood cells, while isoform 2 is the soluble form found in erythrocytes, 23 in African Americans, CYB5R3 is a 301 amino acid protein encoded by the human gene CYB5R3 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CYB5R3 Conjugated |
| Short name: |
FITC Conjugated, CYB5R3 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, CYB5R3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2873 |
| Gene: |
CYB5R3 |
More about : CYB5R3 |
| Long gene name: |
cytochrome b5 reductase 3 |
| Synonyms gene: |
DIA1 |
| Synonyms gene name: |
diaphorase (NADH) (cytochrome b-5 reductase) |
| Synonyms name: |
NADH-cytochrome b5 reductase 3 |
| Locus: |
22q13, 2 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M16461 |
| Entrez gene record: |
1727 |
| Pubmed identfication: |
2479590 3268037 |
| Havana BLAST/BLAT: |
OTTHUMG00000150745 |