| Long name: |
FITC Conjugated, ROCK2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ROCK2 |
| Specificity: |
This is a highly specific antibody against ROCK2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1035-1085/1388 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human ROCK2 |
| Gene ID number: |
9475 |
| Swiss Prot: |
O75116 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation, BRCA2, CNN1, DPYSL2, EP3, EZR, Inhibits keratinocyte terminal differentiation, Involved in regulation of smooth muscle contraction, MSN, MYL9/MLC2, May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles, NPM1, PPP1R12A and VIM, Phosphorylates SORL1 and IRF4, Plays a critical role in the regulation of spine and synaptic properties in the hippocampus, Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation, Plays an important role in the timely initiation of centrosome duplication, Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p9RSK/RPS6KA1 during myogenic differentiation, RDX, actin cytoskeleton organization, cell adhesion and motility via phosphorylation of ADD1, neurite retraction, stress fiber and focal adhesion formation, Protein kinase which is a key regulator of actin cytoskeleton and cell polarity |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ROCK2 Conjugated |
| Short name: |
FITC Conjugated, ROCK2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
coiled-coil containing protein kinase 2 (Antibody to), fluorecein coupled, Rho-associated |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ROCK-II, ROCK2 and IDBG-29657 and ENSG00000134318 and 9475, ROCK2 and IDBG-638012 and ENSBTAG00000005847 and 282041, Rock2 and IDBG-129486 and ENSMUSG00000020580 and 19878, coiled-coil containing protein kinase 2, nuclei, this GO :0000166 and nucleotide binding and molecular function this GO :0000910 and cytokinesis and biological process this GO :0001843 and neural tube closure and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005198 and structural molecule activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006939 and smooth muscle contraction and biological process this GO :0007411 and axon guidance and biological process this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0010825 and positive regulation of centrosome duplication and biological process this GO :0016525 and negative regulation of angiogenesis and biological process this GO :0016772 and transferase activity, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005198 : structural molecule activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005198 : structural molecule activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0017048 : Rho GTPase binding, this GO :0044822 : poly(A) RNA binding, this GO :0046872 : metal ion binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0017048 : Rho GTPase binding and also this GO :0044822 : poly(A) RNA binding and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0017048 and Rho GTPase binding and molecular function this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030155 and regulation of cell adhesion and biological process this GO :0031616 and spindle pole centrosome and cellular component this GO :0032956 and regulation of actin cytoskeleton organization and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0036464 and cytoplasmic ribonucleoprotein granule and cellular component this GO :0042752 and regulation of circadian rhythm and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045616 and regulation of keratinocyte differentiation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048511 and rhythmic process and biological process this GO :0051298 and centrosome duplication and biological process this GO :0051492 and regulation of stress fiber assembly and biological process this GO :0051893 and regulation of focal adhesion assembly and biological process this GO :2000114 and regulation of establishment of cell polarity and biological process this GO :2000145 and regulation of cell motility and biological process, Rho-associated |
| Identity: |
10252 |
| Gene: |
ROCK2 |
More about : ROCK2 |
| Long gene name: |
Rho associated coiled-coil containing protein kinase 2 |
| Locus: |
2p25, 1 |
| Discovery year: |
1999-04-23 |
| GenBank acession: |
D87931 |
| Entrez gene record: |
9475 |
| Pubmed identfication: |
9933571 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000149916 |