| Long name: |
Cy5, 5 Conjugated, UBE3A/E6-AP Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Cy5, 5, 5, Cy5 |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
UBE3A/E6-AP |
| Specificity: |
This is a highly specific antibody against UBE3A/E6-AP |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human UBE3A/E6-AP |
| Gene ID number: |
7337 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Genetic mutations that impair E6-AP activity result in the accumulation of p53 in the cytoplasm, HHR23A, Previous studies have indicated that E6-AP associates with the human papillomavirus E6 oncogene, Several substrates of E6-AP have been identified and include the tumor suppressor protein p53 and the mammalian homolog of Rad23, This conjugation is facilitated by the enzymatic activity of E3 ubiquitin ligase family members such as E6-AP, Ubiquitin (Ub) is directly conjugated to protein substrates by the transfer of Ub from an E2 ubiquitin conjugating enzyme to the target protein, and, in many instances, these mutations are associated with the development of the rare neurodevelopmental disorder Angelman syndrome (AS), which complexes with p53 and thereby potentiates E6-AP mediated ubiquitination of p53, which is characterized by severe motor dysfunction and mental retardation, which selectively targets proteins for degradation by the 26S proteasome, E6-associating protein is a component of the ubiquitin-mediated proteolytic pathway |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
675nm/694nm |
| Synonyms: |
AS ANCR E6-AP FLJ26981 |
| Properties: |
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Conjugated: |
These antibodies are excite for emission at 678nm and detected at a 694 , nm wavelengths |
| Gene target: |
UBE3A/E6-AP Conjugated |
| Short name: |
Conjugated, UBE3A/E6-AP Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
AP |
| Alternative name: |
cyanine 5, 5 coupled, ubiquitin protein ligase E3A/E6-AP (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, ligase activity, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0001541 and ovarian follicle development and biological process this GO :0003713 and transcription coactivator activity and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007420 and brain development and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030521 and androgen receptor signaling pathway and biological process this GO :0035037 and sperm entry and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051865 and protein autoubiquitination and biological process this GO :0060736 and prostate gland growth and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process, this GO :0003713 : transcription coactivator activity, this GO :0003713 : transcription coactivator activity and also this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0016874 : ligase activity, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0005515 : protein binding, this GO :0016874 : ligase activity, 533136, ubiquitin protein ligase E3A |
| Identity: |
12496 |
| Gene: |
UBE3A |
More about : UBE3A |
| Long gene name: |
ubiquitin protein ligase E3A |
| Synonyms gene: |
EPVE6AP HPVE6A |
| Synonyms gene name: |
human papilloma virus E6-associated protein |
| Synonyms name: |
Angelman syndrome |
| Locus: |
15q11, 2 |
| Discovery year: |
1993-10-21 |
| GenBank acession: |
AF002224 |
| Entrez gene record: |
7337 |
| Pubmed identfication: |
8221889 |
| RefSeq identity: |
NM_000462 |
| Havana BLAST/BLAT: |
OTTHUMG00000171548 |
| Locus Specific Databases: |
Manchester LOVD LRG_15 , NGRL |