| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
UBE3A/E6-AP |
| Specificity: |
This antibody reacts specifically with UBE3A/E6-AP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human UBE3A/E6-AP |
| Gene ID: |
7337 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Genetic mutations that impair E6-AP activity result in the accumulation of p53 in the cytoplasm, HHR23A, Previous studies have indicated that E6-AP associates with the human papillomavirus E6 oncogene, Several substrates of E6-AP have been identified and include the tumor suppressor protein p53 and the mammalian homolog of Rad23, This conjugation is facilitated by the enzymatic activity of E3 ubiquitin ligase family members such as E6-AP, Ubiquitin (Ub) is directly conjugated to protein substrates by the transfer of Ub from an E2 ubiquitin conjugating enzyme to the target protein, and, in many instances, these mutations are associated with the development of the rare neurodevelopmental disorder Angelman syndrome (AS), which complexes with p53 and thereby potentiates E6-AP mediated ubiquitination of p53, which is characterized by severe motor dysfunction and mental retardation, which selectively targets proteins for degradation by the 26S proteasome, E6-associating protein is a component of the ubiquitin-mediated proteolytic pathway |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
AS ANCR E6-AP FLJ26981 |
| Also known as: |
UBE3A/E6-AP Polyclonal Antibody |
| Other name: |
Anti-UBE3A/E6-AP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
UBE3A/E6-AP |
| Short name: |
Anti-UBE3A/E6-AP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
AP |
| Alternative name: |
ALEXA Fluor 594, antibody to-ubiquitin protein ligase E3A/E6-AP (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, ligase activity, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0001541 and ovarian follicle development and biological process this GO :0003713 and transcription coactivator activity and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007420 and brain development and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030521 and androgen receptor signaling pathway and biological process this GO :0035037 and sperm entry and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051865 and protein autoubiquitination and biological process this GO :0060736 and prostate gland growth and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process, this GO :0003713 : transcription coactivator activity, this GO :0003713 : transcription coactivator activity and also this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0016874 : ligase activity, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0005515 : protein binding, this GO :0016874 : ligase activity, 533136, ubiquitin protein ligase E3A |
| Identity: |
12496 |
| Gene: |
UBE3A |
More about : UBE3A |
| Long gene name: |
ubiquitin protein ligase E3A |
| Synonyms gene: |
EPVE6AP HPVE6A |
| Synonyms gene name: |
human papilloma virus E6-associated protein |
| Synonyms name: |
Angelman syndrome |
| Locus: |
15q11, 2 |
| Discovery year: |
1993-10-21 |
| GenBank acession: |
AF002224 |
| Entrez gene record: |
7337 |
| Pubmed identfication: |
8221889 |
| RefSeq identity: |
NM_000462 |
| Havana BLAST/BLAT: |
OTTHUMG00000171548 |
| Locus Specific Databases: |
Manchester LOVD LRG_15 , NGRL |