| Long name: |
FITC Conjugated, RAPGEF5/Repac Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RAPGEF5/Repac |
| Specificity: |
This is a highly specific antibody against RAPGEF5/Repac |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human RAPGEF5 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Existing as two alternatively spliced isoforms, Guanine nucleotide exchange factors (GEFs), Repac, The gene that encodes Repac maps to human chromosome 7p15, also known as Rap guanine nucleotide exchange factor 5 (RAPGEF5), is a 580 amino acid protein that is expressed in brain and testis, lung, mouse and rat, pancreas and small intestine, placenta, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation, such as Repac, the Repac gene is conserved in cow, with high expression of this region linking to positive response to erlotinib therapy for those with non-small-cell lung cancer, with weaker expression in heart, 3, Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
KIAA0277 GFR MR-GEF |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RAPGEF5/Repac Conjugated |
| Short name: |
FITC Conjugated, RAPGEF5/Repac Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, RAPGEF5/Repac (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16862 |
| Gene: |
RAPGEF5 |
More about : RAPGEF5 |
| Long gene name: |
Rap guanine nucleotide exchange factor 5 |
| Synonyms gene name: |
Rap guanine nucleotide exchange factor (GEF) 5 |
| Synonyms name: |
M-Ras-regulated GEF |
| Locus: |
7p15, 3 |
| Discovery year: |
2004-03-01 |
| GenBank acession: |
D87467 |
| Entrez gene record: |
9771 |
| Pubmed identfication: |
9039502 10486569 |
| RefSeq identity: |
NM_012294 |
| Havana BLAST/BLAT: |
OTTHUMG00000152525 |