| Long name: |
Biotin Conjugated, RAPGEF5/Repac Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RAPGEF5/Repac |
| Specificity: |
This is a highly specific antibody against RAPGEF5/Repac |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human RAPGEF5 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Existing as two alternatively spliced isoforms, Guanine nucleotide exchange factors (GEFs), Repac, The gene that encodes Repac maps to human chromosome 7p15, also known as Rap guanine nucleotide exchange factor 5 (RAPGEF5), is a 580 amino acid protein that is expressed in brain and testis, lung, mouse and rat, pancreas and small intestine, placenta, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation, such as Repac, the Repac gene is conserved in cow, with high expression of this region linking to positive response to erlotinib therapy for those with non-small-cell lung cancer, with weaker expression in heart, 3, Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
KIAA0277 GFR MR-GEF |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RAPGEF5/Repac Conjugated |
| Short name: |
Conjugated, RAPGEF5/Repac Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, RAPGEF5/Repac (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16862 |
| Gene: |
RAPGEF5 |
More about : RAPGEF5 |
| Long gene name: |
Rap guanine nucleotide exchange factor 5 |
| Synonyms gene name: |
Rap guanine nucleotide exchange factor (GEF) 5 |
| Synonyms name: |
M-Ras-regulated GEF |
| Locus: |
7p15, 3 |
| Discovery year: |
2004-03-01 |
| GenBank acession: |
D87467 |
| Entrez gene record: |
9771 |
| Pubmed identfication: |
9039502 10486569 |
| RefSeq identity: |
NM_012294 |
| Havana BLAST/BLAT: |
OTTHUMG00000152525 |